ABSTRACT
Objective:To study the protective effect of Ficus pandurata extract on acute alcoholic liver injury based on pyroptosis mechanism.Method:The 56 male C57BL/6 mice were randomly divided into normal control group， model control group， positive control group（60 mg·kg-1）， fresh medicine water extract group（48 g·kg-1）， dry drug water extract group（48 g·kg-1），dry drug 50% alcohol extract group（48 g·kg-1） and dry drug 95% alcohol extract group （48 g·kg-1）， 8 mice in each group.Positive control and different solvent extract groups of Ficus tenuifolia were intragastrically administrated for 18 days，once a day，while normal group and model group were given the same volume of pure water intragastrically. After 15 days of continuous gavage， mice received 50％ ethanol（12 mL·kg-1）intragastrically for 3 days to induce acute alcoholic liver injury model except for the normal control group. At 14 h after the last treatment，serum and liver samples were obtained，the serum content of alanine aminotransferase （ALT） and aspartate transaminase（AST） were determined， the histopathologic changes of the hepatic tissues were observed by hematoxylin ecosin（HE） staining.The content of malondialdehyde (MDA) in liver was determined by thiobarbituric acid (TBA) and the content of lactate dehydrogenase (LDH) was determined by microplate method. Western blot and TUNEL assay kit was used to detect the cell pyroptosis rate.Result:Compared with normal group， ALT， AST， MDA and LDH levels in the model group were significantly increased， liver index was significantly increased，TUNEL staining positive， inflammatory factors and pyroptosis related protein expressions were significantly increased （P<0.05）. Compared with model control group， the ALT，AST ，MDA and LDH of the drug intervention group decreased significantly （P<0.05）. The liver index decreased in different degrees， and the expression of inflammatory factors and pyroptosis related protein in the water extract treatment group decreased significantly （P<0.05）.Conclusion:The root extract of Ficus pandurata Hance has protective effect on acute alcoholic liver injury， and the mechanism of water extract might relate to inhibiting hepatocyte pyroptosis.
ABSTRACT
Background Liver is the central hub for metabolism. Liver dysfunction in diabetes mellitus is one of the major causes of morbidity and mortality. Periodical evaluation of transaminases helps in early diagnosis of liver dysfunction. Aim The aim of the present study is to measure aspartate transaminase (AST) and alanine transaminase (ALT) in known cases of type 2 diabetes mellitus (T2DM) and to compare the values with matched controls. Settings and Design Institutional cross-sectional observation study. Methods & Material Study was done in 100 known cases of T2DM and in 30 controls. Age, AST, ALT and fasting plasma glucose (FPG) were recorded, analysed and compared between two groups. Statistical Analysis Data was analysed using Microsoft Excel 2007 and SPSS trial version 16.0. Results Significant difference between FPG, AST, ALT and age were observed between two groups (P < 0.05). Conclusion The results from our study showed that there are elevated levels of ALT and ASTs among T2DM patients when compared with normal individuals.